ISO-CHEK

Simultaneous and Separate Collection of Isocyanate Phases

• Accurately samples diisocyanates: HDI, MDI, IPDI, HMDI, 2,4-TDI, and 2,6-TDI



• The only filter-based system that simultaneously traps and separates both monomers and oligomers
• For better determination of control strategies



• Decreases sample preparation and analysis time by 40% compared to other methods
• Pre-made calibration standards are available



• Highly stable — minimizes storage and handling



• Highly sensitive analysis provides detection limits below current regulated exposure levels
• Ideal for occupational sampling and environmental surveys
• Requires only a 15-minute sample time



• Round-robin proficiency testing for ISO-CHEK-certified laboratories* ensures accurate, consistent analysis



• Meets the specifications of several methods
• ASTM D5932-96 2,4, 2,6-TDI (monomer)
• ASTM D6561-00 HDI (aerosol oligomer), the only U.S. standard for oligomers
• ASTM D6562-00 HDI (gaseous monomer)

ISO-CHEK Isocyanate Sampling System Product Data Bulletin (PDF)

The patented (U.S. Patent No. 4,961,916) ISO-CHEK® Isocyanate Sampling System is a preloaded three-piece cassette that contains two stages. Stage one contains an untreated PTFE (Teflon®) filter to collect the aerosol phase and stage two holds a glass fiber filter impregnated with 9-(N-methylaminomethyl) anthracene (MAMA) for the vapor phase of isocyanates. This system effectively traps and separates the phases at the point of collection and offers many advantages over other methods for sampling isocyanates.

The ISO-CHEK Advantage!

Simultaneous collection and separation of phases at the point of collection: ISO-CHEK separates the vapor and aerosol phases at the point of collection for less time-consuming and more accurate analysis of each phase.

Reagent is stable at room temperature: Loss of derivatizing reagent was found under ambient conditions when using other methods.

15-minute sampling time: Isocyanates are sensitizing agents. Even short 15-minute exposures at high concentrations can lead to sensitivity in susceptible individuals. Therefore, short-term sampling provides more meaningful results than the longer sampling times required by other methods.

No handling precautions: no broken glass or splashing impinger liquid

---

 

Principle of Operation
A sample pump draws air through the ISO-CHEK cassette. Aerosols are trapped on the PTFE filter while gases pass through to the next filter. Vapors are trapped on the fiber glass filter impregnated with MAMA. A chemical reaction occurs on this filter resulting in the formation of a highly detectable urea derivative. The PTFE filter is derivatized in the field by placing it in a supplied jar containing 1-(2 Methoxy phenyl) piperazine reagent (MOPIP). Monomeric and oligomeric phases are separated by using a reverse phase HPLC column with a UV detector and fluorescence detector in series.

Using ISO-CHEK
Simply unplug and connect the cassette to a calibrated sample pump capable of at least 1 L/min. Take a 15-minute sample at 1 L/min. Immediately after sampling, open the cassette, remove the PTFE filter with forceps, match the cassette ID number with the reagent jar number, and place the filter in the prepared reagent jar. The fiber glass filter remains in the cassette. Replug the cassette, wrap it in foil to protect the sample from light, and ship the jar and cassette to an ISO-CHEK-certified laboratory *.

Performance Profile

Collection Method	Dual filter cassette; 5-(m PTFE membrane traps aerosol phase, glass fiber filter impregnated with MAMA collects vapor phase
Sample Flow Rate	1 L/min
Sample Time	15 minutes
Derivatizing Reagent (filter coating for vapor phase)	9-(N-methylaminomethyl) anthracene (MAMA)
Reagent Stability	10 months at ambient temperature
Derivatizing Solution (for aerosol phase)	1-(2-Methoxyphenyl) piperazine (MOPIP)
Sample Stability	Can be stored at 39.2 F (4 C) for up to 10 days. SKC recommends sample analysis as soon as possible.
Analysis	Reverse phase HPLC with UV and fluorescence detection
Quantitation Limit	0.15 ppb for 2,4-TDI, 2,6-TDI, 1,6-HDI, IPDI, MDI, HMDI monomer 1.5 ppb for 1,6-HDI and MDI oligomers
Limit of Detection	0.2 mg/m3 for 2,4-TDI and 2,6-TDI 0.6 mg/m3 for 1,6-HDI 0.3 (UV)/0.08 (FD) mg/m3 ** for MDI

**	First number is with UV detector, the second is with a fluorescence detector

Technical Note Comparison of ISO-CHEK Method Parameters and Reagent Stability (PDF)

Ordering Information           Online Ordering

Description		Quantity	Cat. No.
ISO-CHEK* Sampling System, preloaded clear cassettes for isocyanates Limited shelf life ISO-CHEK cassettes require derivatizing solution (see below) and Sure-Seal Cassette Opener 225-13-5A		12	225-9022
		36	225-9022A
ISO-CHEK* Sampling System with Derivatizing Reagent‡ preloaded clear cassettes and jars of derivatizing solution (MOPIP) Limited shelf life ISO-CHEK cassettes require Sure-Seal Cassette Opener 225-13-5A		4	225-9023
		10	225-9023A
Derivatizing Solution* ‡ , 1-(2 Methoxy phenyl) piperazine reagent (MOPIP), in jars Limited shelf life		12 jars	225-9050
Sure-Seal Cassette Opener, stainless steel, opens 25 and 37-mm cassettes (included in Filter Handling Kit 225-8372) Required for ISO-CHEK cassettes		1	225-13-5A
Jars, glass with PTFE-lined caps, for solvent extraction of filters		8	225-8376
		36	225-8377
ISO-CHEK Calibration Standard Set, calibration standards for HDI, MDI, IPDI, 2,4-TDI, and 2,6-TDI, 1 gram each Limited shelf life. Freezer storage recommended. Does NOT require refrigerated shipping. Other ISO-CHEK standards are available. Contact SKC.		set	225-9055
ISO-CHEK Calibration Standard, MAMA-HMDI, 1 gram Limited shelf life. Freezer storage recommended. Does NOT require refrigerated shipping. Other ISO-CHEK standards are available. Contact SKC.		1	225-9056
ISO-CHEK Calibration Standard, MAMA-2,4 TDI and 2,6 TDI, 1 gram Limited shelf life. Freezer storage recommended. Does NOT require refrigerated shipping. Other ISO-CHEK standards are available. Contact SKC.		1	225-9052
ISO-CHEK Calibration Standard, MAMA-HDI, 1 gram Limited shelf life. Freezer storage recommended. Does NOT require refrigerated shipping. Other ISO-CHEK standards are available. Contact SKC.		1	225-9053
ISO-CHEK Calibration Standard, MAMA-IPDI, 1 gram Limited shelf life. Freezer storage recommended. Does NOT require refrigerated shipping. Other ISO-CHEK standards are available. Contact SKC.		1	225-9054
ISO-CHEK Calibration Standard, MAMA-MDI, 1 gram Limited shelf life. Freezer storage recommended. Does NOT require refrigerated shipping. Other ISO-CHEK standards are available. Contact SKC.		1	225-9062
ISO-CHEK Packaging Kit, provides shipping material to make 10 packaging kits each holding 10 samplers and derivatizing solution jars		1	225-9059
ISO-CHEK Packaging Kit includes shipping material to make 20 packages each holding 4 ISO-CHEK samplers and 4 derivatizing solution jars		1	225-9063

*	ISO-CHEK cassettes must be analyzed by an ISO-CHEK-certified laboratory. For a lab list see the SKC ISO-CHEK Cassettes Laboratory Reference List.
‡	Hazmat shipping charges for air shipments only, ground shipments exempt; not available for export
†	IRSST (Institut de recherche Robert-Sauveé en santé et en sécurité du travail du Québec)

ISO-CHEK Isocyanate Sampling System Product Data Bulletin (PDF)

Material Safety Data Sheets

MSDS — MOPIP 1-(2-Methoxyphenyl) Piperazine (PDF)

MSDS — MAMA 9-(Methylaminomethyl) Anthracene (PDF)

MSDS — Toluene (PDF)

® ISO-CHEK is a registered trademark of Omega Specialty Instrument Co.

---

This page was last modified March 8, 2010