SKC: Shaping the Future of Air Sampling  

 

 

Gelatin Filters

Preserve Viability of Microbes

  • Unequaled bacteria retention levels as high as 99.9995% for Bacillus subtilis niger
  • Sterilized with gamma radiation
  • Designed specifically for the collection of and analysis of airborne microbes
  • High moisture content helps to maintain microorganism viability for sampling periods up to 1 hour
  • Help increase survival of stress-sensitive microbes
  • Suitable for isokinetic sampling of both bacteria and viruses
  • Simple analysis techniques
  • Dissolve easily when placed on agar
Gelatin Filters Instructions (PDF)

Gelatin Filters Product Data Bulletin (PDF)

Gelatin filters reliably retain bacteria and viruses and are routinely used to collect airborne microorganisms for quantitative analysis. The gelatin's inherent high-moisture content helps to maintain viability of stress-sensitive microorganisms for sampling periods up to 1 hour. Gelatin filters dissolve easily when placed on agar, allowing a gentle transition from sample medium to growth medium. Refrigerator storage (4 to 8 C) is recommended. Avoid exposure to temperatures below 4 C, moisture, and chemical vapors.

Filter sampling for bioaerosols is easy, efficient, and can provide information about relative changes in bioaerosol concentration throughout the day. However, filter sampling of less hardy microorganisms can reduce culturability due to desiccation. The use of gelatin filters can help to maintain viability. For maximum culturability and superior collection of inhalable-size bioaerosols, combine gelatin filters with the SKC Button Sampler. See below.

Performance Profile

Material Water-soluble gelatin
Pore Size While having a nominal pore size of 3.0 µm, a higher capture efficiency of sub-micron particles can be expected due to the separations that occur on the surface and within the filter. It is through inertial impaction and diffusional interception that these filters can remove particles much smaller that 3.0 µm.
Diameter 25 mm or 37 mm
Thickness Approximately 250 µm
Sterilization Presterilized by gamma radiation. Filters individually wrapped and packaged in units of five each in a polyethylene bag.
Thermal Resistance Maximum 140 F (60 C)
Residual Dampness Content 46 to 49%
Conditions for Use Maximum room temperature is 86 F (30C); relative humidity is 85%
Sampling Time Up to 1 hour at typical indoor relative humidity
Sampling Flow Rate

1 to 4 L/min for filter sampling of biological contaminants using a standard filter cassette, Button Sampler, or the IOM

Storage Gelatin filters may be stored in low humidity, ambient conditions; storage between 39.2 and 46.4 F (4 and 8 C) is recommended.
Caution: Do not store gelatin filters below 39.2 F (4 C). Condensation during thawing will dissolve filter. Avoid exposing filters to moisture, chemical vapors, and extreme temperatures.
Shelf-life 3 years from date of manufacture
Analysis Direct method or indirect method. Gelatin filters dissolve when placed on agar plates.

Ordering Information    
Diameter
(mm)
Support Pad Included
Notes
Qty.
Cat. No.
25 No Water-soluble gelatin, presterilized, individually wrapped, packaged in units of five each in a polyethylene bag
Recommended for use with the Button Sampler and IOM
Limited shelf-life; refrigerated storage from 39.2 to 46.4 F (4 to 8 C) recommended
50
37 No Water-soluble gelatin, presterilized, individually wrapped, packaged in units of five each in a polyethylene bag
Recommended for use with the Button Sampler and IOM
Limited shelf-life; refrigerated storage from 39.2 to 46.4 F (4 to 8 C) recommended
50


Gelatin Filters Instructions (PDF)

Gelatin Filters Product Data Bulletin (PDF)


About Using Gelatin Filters with the Button Sampler


For maximum microorganism survivability and superior collection of inhalable-size bioaerosols, use sterile gelatin filters with the SKC Button Sampler. The Button Sampler features a unique inlet that contains evenly spaced holes that act as sampling orifices for multi-directional sampling. The proximity of the filter to the inlet minimizes transmission losses and provides equal distribution of particle loading and low intersample variation. Combining the Button Sampler with the nurturing properties of gelatin filters creates a sampler that is most efficient at collecting inhalable bioaerosols for viable or non-viable analysis. Click here to go to the Button Sampler Page.

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A typical filter will have a rough side and a shiny side. The shiny side is easier to read under a microscope, therefore, expose the shiny side to the air for microscopic analysis. The rough side holds dust more effectively; expose the rough side for gravimetric analysis.

Gelatin filters feature high moisture content, however, they are very brittle and break easily. Handle with care.


This page was last modified June 10, 2010
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