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![]() | The SKC BioStage® viable cascade impactor meets NIOSH requirements and ACGIH recommendations for sampling indoor and outdoor mold and bacteria. The BioStage comprises an inlet cone, precision-drilled 400-hole impactor stage, and a base that holds a standard-size agar plate. A high flow pump, such as the QuickTake 30, pulls microorganisms in air through the holes (jets) where they are collected on the agar surface. Testing demonstrates that BioStage provides performance equivalent to the industry-standard Andersen N-6. What sets BioStage apart from other samplers is its SureLock positive seal (instead of bulky spring clamps) that ensures sample integrity. |
• | Remove O-rings prior to autoclaving. |
** | Samimi, B. and Shufutinsky, A., "Comparison of the Thermo-Andersen N6, the Aerotech A6, the SKC BioStage, and the SKC Micromedia Viable Samplers in the Collecting Airborne Fungal Spores," AIHce, San Diego, CA, Final Program, p. 43 |
Flow Rate
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28.3 L/min |
Material
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Inlet cone and base plate: Precision-tooled
autoclavable aluminum O-rings: Duro 50, BUNA-N (not autoclavable ) |
Jet Classification Stage
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400 holes (0.25-mm hole diameter |
Median Cut-point (D50) | 0.6 µm |
Sample Media
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90 to 100-mm agar plates* |
Suggested Media
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For bacteria: Tryptic Soy Agar (TSA) Blood Agar Plates (BAP) For fungi (viable): Potato Dextrose Agar (PDA) Malt Extract Agar (MEA) Dichloran Glycerol 18 Agar (DG-18) Corn Meal Agar (CMA) |
Analysis
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Colony culture‡ |
Tubing | 1/4-inch ID |
* | Requires microbiological media supplied by analytical laboratories. For lab list see the: BioStage Analytical Laboratories Reference List or visit the AIHA website at www.aiha.org. |
‡ | In situations where spore counts are high, positive hole correction should be used. See Macher, J., "Positive-Hole Correction of Multiple-jet Impactors for Collecting Viable Microorganisms," American Industrial Hygiene Journal, 50 (11), 1989, pp. 561-568 |
The BioStage single-stage cascade impactor operates on the principle of inertial impaction and meets NIOSH and ACGIH recommendations for sampling indoor and outdoor air for mold and bacteria. Made of precision-tooled aluminum, the sampler contains a jet classification stage and base plate precision-threaded together for a positive seal and an inlet cone. The impactor stage contains precision-drilled holes. A sample pump draws air through the sampler where multiple jets of air direct airborne particles toward the surface of the agar collection medium.
Only SKC offers the SureLock positive seal advantage! The SKC BioStage Cascade Impactor is constructed with the same 400-hole jet classification stage as the Andersen N-6 Impactor. However, only the SKC BioStage features the SureLock positive seal that:
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with spring clamps |
with SureLock Seal |
The QuickTake 30 The SKC QuickTake 30 Sample Pump is designed for easy use with the SKC BioStage and other impaction devices that require flows to 30 L/min - it provides the constant 28.3 L/min flow rate for the BioStage. The mounting bracket accessory holds the BioStage Impactor securely onto the QuickTake 30 sample pump for fast setup, easy sampling, and portability. |
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** | Samimi, B. and Shufutinsky, A., "Comparison of the Thermo-Andersen N6, the Aerotech A6, the SKC BioStage, and the SKC Micromedia Viable Samplers in the Collecting Airborne Fungal Spores," AIHce, San Diego, CA, Final Program, p. 43 |
Yao, M. and Mainelis, G., "Analysis of Portable Impactor Performance for Enumeration of Viable Bioaerosols," Journal of Occupational and Environmental Hygiene, Vol. 4, Issue 7, July 2007, pp. 514-524 | |
Dobranic, J., Superbugs in Our Communities – An Introduction for the IEQ Professional, EMSL. Presented at 2008 IAQ Conference, Tampa, FL |